Recombinant Human PPM1A, His-tagged

• Cat.No.: PPM1A-31047TH
• Product Overview: Recombinant fusion protein, corresponding to amino acids 1-382 of Human PPM1A (full-length), with N-terminal His tag, T7 gene 10 leader, XpressTM Epitope.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 1-382 a.a.
• Description: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.
• Conjugation: HIS
• Biological activity: Specific activity: >8,000units/mg of PPM1A.Unit Definition - one unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37°C using 10mM of substrate.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 50mM Sodium chloride, 2mM DTT, 10mM Tris HCl, 1mM Manganese chloride, pH 7.5
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
• Sequence Similarities: Belongs to the PP2C family.

Gene Information

• Gene Name: PPM1A protein phosphatase, Mg2+/Mn2+ dependent, 1A [ Homo sapiens ]
• Official Symbol: PPM1A
• Synonyms: PPM1A; protein phosphatase, Mg2+/Mn2+ dependent, 1A; protein phosphatase 1A (formerly 2C), magnesium dependent, alpha isoform; protein phosphatase 1A; MGC9201; phosphatase 2C alpha; PP2CA; PP2Calpha; protein phosphatase 2C; alpha isoform
• Gene ID: 5494
• mRNA Refseq: NM_021003
• Protein Refseq: NP_066283
• MIM: 606108
• Uniprot ID: P35813
• Chromosome Location: 14q23.1
• Pathway: BMP receptor signaling, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Insulin receptor signalling cascade, organism-specific biosystem
• Function: R-SMAD binding; hydrolase activity; magnesium ion binding; manganese ion binding; protein C-terminus binding