Recombinant Human POU5F1 protein, His-tagged

Description :	POu domain, class 5, transcription factor 1, also known as OCT4, binds to the octamer motif (5'-ATTTGCAT-3'). This protein forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, uTF1 and ZFP206. It is critical for early embryogenesis and for embryonic stem cell pluripotency.
Source :	E. coli
Species :	Human
Tag :	His
Form :	Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Molecular Mass :	32.2 kDa (285aa)
AA Sequence :	MGSSHHHHHH SSGLVPRGSH MHFYRLFLGA TRRFLNPEWK GEIDNWCVYV LTSLLPFKIQ SQDIKALQKE LEQFAKLLKQ KRITLGYTQADVGLTLGVLF GKVFSQTTIC RFEALQLSFK NMCKLRPLLQ KWVEEADNNE NLQEICKAET LVQARKRKRT SIENRVRGNL ENLFLQCPKPTLQQISHIAQ QLGLEKDVVR VWFCNRRQKG KRSSSDYAQR EDFEAAGSPF SGGPVSFPLA PGPHFGTPGY GSPHFTALYS SVPFPEGEAFPPVSVTTLGS PMHSN
Purity :	> 85% by SDS - PAGE
Storage :	Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration :	0.5 mg/ml (determined by Bradford assay)
Protein length :	1-265 a.a.

Gene Name	POU5F1 POU class 5 homeobox 1 [ Homo sapiens ]
Official Symbol	POU5F1
Synonyms	POU5F1; POU class 5 homeobox 1; OTF3, POU domain class 5, transcription factor 1; POU domain, class 5, transcription factor 1; MGC22487; OCT3; Oct4; octamer-binding protein 3; octamer-binding protein 4; POU domain transcription factor OCT4; octamer-binding transcription factor 3; octamer-binding transcription factor-3; POU-type homeodomain-containing DNA-binding protein; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4;
Gene ID	5460
mRNA Refseq	NM_203289
Protein Refseq	NP_976034
MIM	164177
UniProt ID	Q01860
Chromosome Location	6p21.33
Pathway	HIF-2-alpha transcription factor network, organism-specific biosystem; Wnt Signaling Pathway and Pluripotency, organism-specific biosystem;
Function	DNA binding; miRNA binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding RNA polymerase II transcription factor activity; sequence-specific DNA binding transcription factor activity; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding;