Recombinant Human Polymerase (DNA directed), Lambda, His-tagged

• Cat.No.: POLL-6891H
• Product Overview: Recombinant human POLL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. MW: 36 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-300aa
• Description: POLL is a DNA polymerase involved in base excision repair (BER). POLL is a low-fidelity polymerase which plays a role in both spontaneous and DNA damage-induced mutagenesis. In addition, POLL may play a role in the mutagenic bypass of T-T dimers. Pol lambda possesses terminal transferase and 5'-deoxyribose-5-phosphate lyase activity. These activities have been found to be coordinated by PCNA and replication protein A.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl.
• Molecular Mass: 36 kDa (320aa), confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HRRLDIIVVP YSEFACALLY FTGSAHFNRS MRALAKTKGM SLSEHALSTA VVRNTHGCKV GPGRVLPTPT EKDVFRLLGL PYREPAERDW
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: POLL polymerase (DNA directed), lambda [ Homo sapiens ]
• Official Symbol: POLL
• Synonyms: POLL; polymerase (DNA directed), lambda; DNA polymerase lambda; DNA polymerase kappa; DNA polymerase beta-2; DNA polymerase beta-N; BETAN; POLKAPPA; FLJ46002
• Gene ID: 27343
• mRNA Refseq: NM_001174084
• Protein Refseq: NP_001167555
• MIM: 606343
• UniProt ID: Q9UGP5
• Chromosome Location: 10q23
• Pathway: BER complex, organism-specific biosystem; BER complex, conserved biosystem; Base excision repair, organism-specific biosystem; Base excision repair, conserved biosystem; Non-homologous end-joining, organism-specific biosystem; Non-homologous end-joining, conserved biosystem
• Function: DNA binding; DNA binding; DNA-directed DNA polymerase activity; DNA-directed DNA polymerase activity; catalytic activity; lyase activity; metal ion binding; nucleotidyltransferase activity; transferase activity