Recombinant Human POLR2I, His-tagged

• Cat.No.: POLR2I-522H
• Product Overview: Recombinant Human POLR2I with His tag is expressed inE. colithat carries the coding sequence of human RPB9 under the control of a T7 promoter.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: hRPB9 is a subunit unique to RNA polymerase II, although it has sequence homologues in RNA polymerases I and III. The gene for Rpb9 is not essential for yeast cell viability, but is essential in Drosophila. hRPB9 has roles in both transcription initiation and trascription elongation. In the initiation reaction it is necessary for accurate start site selection. In the elongation reaction, RPB9, along with TFIIS facilitates the conversion of an arrest-competent conformation to a read-through competent conformation.
• Purity: Purity > 95% bySDS-PAGE analysis.
• Application: RPB5 has been applied in protein-protein interactions assays.
• Endotoxin: 1 unit equals 1 nanogram of purified protein.
• Buffer: 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.

Gene Information

• Gene Name: POLR2I polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa [ Homo sapiens ]
• Synonyms: POLR2I; polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa; DNA-directed RNA polymerase II 14.5 kDa polypeptide; DNA-directed RNA polymerase II subunit I; DNA-directed RNA polymerase II subunit RPB9; RNA polymerase II subunit B9; RPB14.5; RPB9; hRPB14.5; POLR2I
• Gene ID: 5438
• mRNA Refseq: NM_006233
• Protein Refseq: NP_006224
• MIM: 180662
• UniProt ID: P36954
• Chromosome Location: 19q12
• Pathway: Huntington"s disease; Metabolic pathways; Purine metabolism; RNA polymerase; Gene Expression; Regulatory RNA pathways; mRNA Processing
• Function: DNA binding; DNA-directed RNA polymerase activity; protein binding; protein kinase activity; zinc ion binding