Recombinant Human POLR2H, GST-tagged

• Cat.No.: POLR2H-8509H
• Product Overview: Recombinant Human POLR2H,fused with GST tag,was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Description: The RPB8 subunit is present in all three types of eukaryotic RNA polymerases and is highly conserved during evolution. It is an essential subunit required for the transcription of nuclear genes. Rpb8 interacts with the largest subunit Rpb1, and with Rpb3 and Rpb11, two smaller subunits. hRPB8 interacts with single-stranded DNA nonspecifically. When DNA is damaged in cells, the breast and ovarian tumor suppressor BRCA1 ubiquitinates RPB8, which may relate with the BRCA1-dependent cell survival.
• Form: 20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
• AA Sequence: MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFEY VMYGKVYRIE GDETSTEAAT RLSAYVSYGG LLMRLQGDAN NLHGFEVDSR VYLLMKKLAF
• Purity: >95% as determined by SDS-PAGE
• Storage: -80 °C
• Concentration: 300 μg/ml
• Shipping: Dry Ice

Gene Information

• Gene Name: POLR2H polymerase (RNA) II (DNA directed) polypeptide H [ Homo sapiens ]
• Official Symbol: POLR2H
• Synonyms: POLR2H; polymerase (RNA) II (DNA directed) polypeptide H; DNA-directed RNA polymerases I, II, and III subunit RPABC3; RPB8; hRPB8; RPB8 homolog; DNA-directed RNA polymerase II subunit H; RNA polymerases I, II, and III subunit ABC3; DNA-directed RNA polymerases I, II, and III 17.1 kDa polypeptide; RPB17; RPABC3; hsRPB8
• Gene ID: 5437
• mRNA Refseq: NM_006232
• Protein Refseq: NP_006223
• MIM: 606023
• UniProt ID: P52434
• Chromosome Location: 3q28
• Pathway: Abortive elongation of HIV-1 transcript in the absence of Tat, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; DNA Repair, organism-specific biosystem; Disease, organism-specific biosystem; Dual incision reaction in TC-NER, organism-specific biosystem; Eukaryotic Transcription Initiation, organism-specific biosystem
• Function: DNA-directed RNA polymerase activity; contributes_to protein kinase activity; zinc ion binding