Recombinant Human PODXL protein, His & GST-tagged

• Cat.No.: PODXL-7641H
• Product Overview: Recombinant Human PODXL aa. (Ala220~Ile431 (Accession # O00592)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Ala220~Ile431
• Description: This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 55.1kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.2.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PODXL podocalyxin like [ Homo sapiens (human) ]
• Official Symbol: PODXL
• Synonyms: PC; PCLP; Gp200; PCLP-1; GCTM-2 antigen; podocalyxin-like protein 1; podocalyxin
• Gene ID: 5420
• mRNA Refseq: NM_001018111.2
• Protein Refseq: NP_001018121.1
• UniProt ID: O00592