Recombinant Human PLOD2 protein, His & T7-tagged

• Cat.No.: PLOD2-1181H
• Product Overview: Recombinant Human PLOD2 aa. (Lys644~Pro737 (Accession # O00469) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Lys644~Pro737
• Description: The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 14.3kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PLOD2 procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 [ Homo sapiens (human) ]
• Official Symbol: PLOD2
• Synonyms: PLOD2; procollagen-lysine,2-oxoglutarate 5-dioxygenase 2; LH2; TLH; BRKS2; procollagen-lysine,2-oxoglutarate 5-dioxygenase 2; lysine hydroxylase 2; lysyl hydroxlase 2; lysyl hydroxylase 2; procollagen-lysine 5-dioxygenase; telopeptide lysyl hydroxylase
• Gene ID: 5352
• mRNA Refseq: NM_000935.2
• Protein Refseq: NP_000926.2
• UniProt ID: O00469