Recombinant Human PLIN2 Protein (444 AA), N-8×His tagged

Cat.No. : PLIN2-23H
Product Overview : Recombinant Human PLIN2 Protein with N-8×His-tag was expressed in E. coli.
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Species : Human
Source : E.coli
Tag : His
Protein Length : 444 AA
Description : The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
Form : Lyophilized
Molecular Mass : 49,03 kDa (calculated)
AA Sequence : MKHHHHHHASVAVDPQPSVVTRVVNLPLVSSTYDLMSSAYLSTKDQYPYLKSVCEMAENGVKTITSVAMTSALPIIQKLEPQIAVANTYACKGLDRIEERLPILNQPSTQIVANAKGAVTGAKDAVTTTVTGAKDSVASTITGVMDKTKGAVTGSVEKTKSVVSGSINTVLGSRMMQLVSSGVENALTKSELLVEQYLPLTEEELEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVKEAKQKSQQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDDTDESHCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKHMGVMAGDIYSVFRNAASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVNNTPLNWLVGPFYPQLTESQNAQDQGAEMDKSSQETQRSEHKTH
Purity : > 95%
Applications : WB
Quality Control Test : BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Notes : This product is intended for research use only.
Storage : Store the lyophilized protein at –80 centigrade. Lyophilized protein remains stable until the expiry date when stored at –80 centigrade. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 centigrade for long term storage. Reconstituted protein can be stored at 4 centigrade for a week.
Concentration : 0.5 mg/mL
Storage Buffer : Filtered (0.4 μm) in 20mM Tris buffer, 20mM NaCl, pH 7.5
Reconstitution : Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
Shipping : At ambient temperature.
Gene Name PLIN2 perilipin 2 [ Homo sapiens (human) ]
Official Symbol PLIN2
Synonyms PLIN2; perilipin 2; ADFP; ADRP; perilipin-2; adipophilin; adipose differentiation-related protein
Gene ID 123
mRNA Refseq NM_001122
Protein Refseq NP_001113
MIM 103195
UniProt ID Q99541

Not For Human Consumption!

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