Recombinant Human PLIN2 Protein (444 AA), N-8×His tagged

• Cat.No.: PLIN2-23H
• Product Overview: Recombinant Human PLIN2 Protein with N-8×His-tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Lyophilized
• Molecular Mass: 49,03 kDa (calculated)
• Protein length: 444 AA
• AA Sequence: MKHHHHHHASVAVDPQPSVVTRVVN LPLVSSTYDLMSSAYLSTKDQYPYL KSVCEMAENGVKTITSVAMTSALPI IQKLEPQIAVANTYACKGLDRIEER LPILNQPSTQIVANAKGAVTGAKDA VTTTVTGAKDSVASTITGVMDKTKG AVTGSVEKTKSVVSGSINTVLGSRM MQLVSSGVENALTKSELLVEQYLPL TEEELEKEAKKVEGFDLVQKPSYYV RLGSLSTKLHSRAYQQALSRVKEAK QKSQQTISQLHSTVHLIEFARKNVY SANQKIQDAQDKLYLSWVEWKRSIG YDDTDESHCAEHIESRTLAIARNLT QQLQTTCHTLLSNIQGVPQNIQDQA KHMGVMAGDIYSVFRNAASFKEVSD SLLTSSKGQLQKMKESLDDVMDYLV NNTPLNWLVGPFYPQLTESQNAQDQ GAEMDKSSQETQRSEHKTH
• Purity: > 95%
• Applications: WB
• Quality Control Test: BCA to determine quantity of the protein.
  SDS PAGE to determine purity of the protein.
• Notes: This product is intended for research use only.
• Storage: Store the lyophilized protein at –80 centigrade. Lyophilized protein remains stable until the expiry date when stored at –80 centigrade. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 centigrade for long term storage. Reconstituted protein can be stored at 4 centigrade for a week.
• Concentration: 0.5 mg/mL
• Storage Buffer: Filtered (0.4 μm) in 20mM Tris buffer, 20mM NaCl, pH 7.5
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.
• Shipping: At ambient temperature.

Gene Information

• Gene Name: PLIN2 perilipin 2 [ Homo sapiens (human) ]
• Official Symbol: PLIN2
• Synonyms: PLIN2; perilipin 2; ADFP; ADRP; perilipin-2; adipophilin; adipose differentiation-related protein
• Gene ID: 123
• mRNA Refseq: NM_001122
• Protein Refseq: NP_001113
• MIM: 103195
• UniProt ID: Q99541

Reviews

01/15/2022

Operation is simple and does not require complex operation steps.

10/06/2021

Quality I use is very good and I have recommended this product to my colleagues and friends.

01/24/2021

Sensitivity in Western blot is very high and low concentrations of the target protein can be detected.

Q&As

How are the interactions between PLIN2 and other proteins regulated?

The interaction between PLIN2 and other proteins is regulated by the formation of complexes or the regulation of signal transduction pathways. These interactions can be influenced by a variety of factors, such as nutrients, hormones, and inflammatory factors.

What is the relationship between PLIN2 and cardiovascular disease?

Some studies have suggested that the expression level of PLIN2 may be associated with the occurrence and progression of cardiovascular disease. High levels of PLIN2 may be associated with an increased risk of cardiovascular diseases such as atherosclerosis, myocardial hypertrophy, and heart failure.

Can the function of PLIN2 be studied by gene knockout or knockdown techniques?

The function of PLIN2 can be studied through gene knockout or knockdown techniques, such as using techniques such as CRISPR-Cas9 or RNAi. These techniques can create cellular models of PLIN2 deletion or reduction to observe its effects on fat storage and breakdown, among other things.

What is the role of PLIN2 in obesity and diabetes?

PLIN2 plays an important role in obesity and diabetes. It may influence the development of obesity and diabetes by regulating adipocyte differentiation, insulin sensitivity, and lipid metabolism.

How can the function of PLIN2 be studied by overexpression techniques?

PLIN2 can be studied through overexpression techniques, such as the use of gene transduction or viral transduction. These techniques can increase the expression level of PLIN2 to observe its effects on fat storage and breakdown, among other things.

How does the expression level of PLIN2 relate to the occurrence and progression of the disease?

Some studies have shown that PLIN2 expression levels may be associated with obesity, diabetes, and cardiovascular disease, among others. High levels of PLIN2 may be associated with increased susceptibility to disease, while low levels of PLIN2 may be associated with decreased susceptibility to disease.