Recombinant Human PLCG2 protein, His & T7-tagged

• Cat.No.: PLCG2-1018H
• Product Overview: Recombinant Human PLCG2 aa. (Leu930~Thr1152 (Accession # P16885) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: His&T7
• Protein Length: Leu930~Thr1152
• Description: The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.3kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.8.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PLCG2 phospholipase C gamma 2 [ Homo sapiens (human) ]
• Official Symbol: PLCG2
• Synonyms: PLCG2; phospholipase C gamma 2; FCAS3; APLAID; PLC-IV; PLC-gamma-2; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; phosphoinositide phospholipase C-gamma-2; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C-IV
• Gene ID: 5336
• mRNA Refseq: NM_002661.4
• Protein Refseq: NP_002652.2
• UniProt ID: P16885