Active Recombinant Human PLCG2 protein, Myc/DDK-tagged

• Cat.No.: PLCG2-203H
• Product Overview: Recombinant protein of human phospholipase C, gamma 2 (phosphatidylinositol-specific) (PLCG2) with a C-Myc/DDK tag was expressed in HEK293T.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3.
• Bio-activity: Measured by its binding ability in a functional ELISA. When rhvWF-A2 is coated at 1 μg/mL (100 μL/well), the concentration of rhGPV that produces 50% optimal binding response is found to be approximately 10-50 ng/mL. Measured by the ability of the immobilized protein to support the adhesion of thrombin-activated human platelets.
  When 5 x 10^6 cells per well are added to rhGPV coated plates (5 μg/mL, 100 μL/well), approximately 50%-70% will adhere after 1 hour at 37 centigrade.
  Optimal dilutions should be determined by each laboratory for each application.
• Molecular Mass: 147.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol

Gene Information

• Gene Name: PLCG2 phospholipase C gamma 2 [ Homo sapiens (human) ]
• Official Symbol: PLCG2
• Synonyms: PLCG2; phospholipase C gamma 2; FCAS3; APLAID; PLC-IV; PLC-gamma-2; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2; phosphoinositide phospholipase C-gamma-2; phospholipase C, gamma 2 (phosphatidylinositol-specific); phospholipase C-IV; EC 3.1.4.11
• Gene ID: 5336
• mRNA Refseq: NM_002661
• Protein Refseq: NP_002652
• MIM: 600220
• UniProt ID: P16885