Recombinant Human PLAU protein, His-tagged

• Cat.No.: PLAU-314H
• Product Overview: Recombinant Human PLAU (Ser21-Leu431) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Ser21
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Sodium Acetate, 100 mM NaCl, pH5.0, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 45.0 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ser21-Leu431
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >92% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PLAU
• Official Symbol: PLAU
• Synonyms: PLAU; plasminogen activator, urokinase; urokinase-type plasminogen activator; UPA; URK; U-plasminogen activator; plasminogen activator, urinary; ATF; QPD; u-PA; BDPLT5
• Gene ID: 5328
• mRNA Refseq: NM_001145031
• Protein Refseq: NP_001138503
• MIM: 191840
• UniProt ID: P00749