Recombinant Human Platelet Derived Growth Factor C

Cat. No. :	PDGFC-441H
Description :	The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14 kDa polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-α and PDGFR-β, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet α-granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signa primarily through the PDGF-Rα receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-Rβ receptor. Recombinant human PDGF-CC is a 25kDa protein consisting of two identical disulfide-linked 114 amino-acid polypeptide chains.
Source :	E. coli.
Molecular Weight :	25 kDa.
Endotoxin Level :	< 0.1 ng per µg of rHu PDGF-CC (1EU/µg).
Purity :	Greater than 98% (determined by SDS-PAGE and HPLC analysis).
Biological Activity :	Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED₅₀for this effect is 15-20 ng/ml.
Formulation :	Lyophilized from a sterile-filtered solution with no additives.
Reconstitution :	Please Note: Always centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage/Stability :	The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Tag :	Non

Gene Name	PDGFC platelet derived growth factor C [ Homo sapiens ]
Synonyms	platelet derived growth factor C; SCDGF; FALLOTEIN; PDGFC; spinal cord-derived growth factor; secretory growth factor-like protein; fallotein; fallotein; PDGF-C; VEGF-E; Spinal cord-derived growth factor; platelet-derived growth factor C
Gene ID	56034
mRNA Refseq	NM_016205
Protein Refseq	NP_057289
MIM	608452
UniProt ID	Q9NRA1
Chromosome Location	4q32
Pathway	Cytokine-cytokine receptor interaction; Regulation of actin cytoskeleton; Focal adhesion; Gap junction; Melanoma; Prostate cancer; Signaling by PDGF; Signaling by VEGF
Function	platelet-derived growth factor receptor binding; protein homodimerization activity; growth factor activity; cell surface binding