Active Recombinant Human PDGFC Protein, Met & His-tagged

• Cat.No.: PDGFC-699H
• Product Overview: Recombinant Human PDGFC (Accession # NP_057289) Val235-Gly345, fused with an N-terminal Met and 6-His tag, was produced in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Val235-Gly345
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 10^9:749.The ED50 for this effect is typically 70 - 350 ng/mL.
• Molecular Mass: Recombinant Human PDGFC, Met & His-tagged has a calculated MW of 13.4 kDa (monomer).
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in PBS.

Gene Information

• Gene Name: PDGFC platelet derived growth factor C [ Homo sapiens ]
• Official Symbol: PDGFC
• Synonyms: PDGFC; platelet derived growth factor C; platelet-derived growth factor C; fallotein; SCDGF; PDGF-C; VEGF-E; spinal cord-derived growth factor; secretory growth factor-like protein; FALLOTEIN
• Gene ID: 56034
• mRNA Refseq: NM_016205
• Protein Refseq: NP_057289
• MIM: 608452
• UniProt ID: Q9NRA1
• Chromosome Location: 4q32