Recombinant Human Plasminogen

• Cat.No.: PLG-66H
• Product Overview: Recombinant Human Angiostatin produced inPichia Pastorisis a single, non-glycosylated polypeptide chain having a molecular mass of 29711 Dalton.

Specification

• Species: Human
• Source: P.pastoris
• Tag: Non
• Description: RHuAng K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active relative to kringles 1-4. rHuAng K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. The rHuAng K1-3 is purified by proprietary chromatographic techniques.
• Physical Appearance: Sterile Filtered lyophilized powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Formulation: Lyophilized from a (1mg/ml) solution in containing 0.5X PBS.
• Solubility: It is recommended to reconstitute the lyophilized rHuAng K1-3 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: Inhibitory activity (ED₅₀=70 nm) relative to Kringles 1-4 (ED50=135 nm). Kringles 1-3 acts as inhibitor of endothelial cell growth and angiogenesis.
• Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuAng K1-3.
• Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.08 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of rHuAng K1-3 as a Reference Standard.
• Storage: Lyophilized rHuAng K1-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuAng K1-3 should be stored at 4°Cbetween 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PLG plasminogen [ Homo sapiens ]
• Synonyms: PLG; plasminogen; DKFZp779M0222; Plasminogen; Plasmin heavy chain A;Activation peptide; Angiostatin; Plasmin heavy chainA, short form; Plasmin light chain B;EC 3.4.21.7; OTTHUMP00000017544
• Gene ID: 5340
• mRNA Refseq: NM_000301
• Protein Refseq: NP_000292
• MIM: 173350
• UniProt ID: P00747
• Chromosome Location: 6q26
• Pathway: Complement and coagulation cascades; Neuroactive ligand-receptor interaction; Diabetes pathways; Hemostasis; Signaling by PDGF
• Function: calcium ion binding; apolipoprotein binding; peptidase activity; serine-type endopeptidase activity