Recombinant Human PKM, MYC/DDK-tagged

• Cat.No.: PKM-68H
• Product Overview: Recombinant Human pyruvate kinase, muscle (PKM2), transcript variant 1, fused with C-terminal MYC/DDK, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
• Molecular Mass: 57.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: PKM pyruvate kinase, muscle [ Homo sapiens (human) ]
• Official Symbol: PKM
• Synonyms: PKM; pyruvate kinase, muscle; PK3; PKM; TCB; OIP3; CTHBP; THBP1; MGC3932; Pyruvate kinase isozymes M1/M2; EC 2.7.1.40; Pyruvate kinase muscle isozyme; Pyruvate kinase 2/3; Cytosolic thyroid hormone-binding protein; Cytosolic thyroid hormone-bin ding protein; OPA-interacting protein 3; PK, muscle type; pyruvate kinase, muscle; thyroid hormone-binding protein, cytosolic
• Gene ID: 5315
• mRNA Refseq: NM_002654
• Protein Refseq: NP_002645
• MIM: 179050
• UniProt ID: P14618
• Chromosome Location: 15q22
• Pathway: Adenine ribonucleotide biosynthesis, IMP => ADP,ATP; Biosynthesis of amino acids; Glycogen storage diseases
• Function: ATP binding; MHC class II protein complex binding; magnesium ion binding