Recombinant Human PKLR, His-tagged

• Cat.No.: PKLR-30455TH
• Product Overview: Recombinant fragment corresponding to amino acids 47-574 of Human PKLR with an N terminal His tag; 549 amino acids with tag, Predicted MWt 59.2 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• ProteinLength: 528 amino acids
• Description: The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
• Conjugation: HIS
• Molecular Weight: 59.200kDa inclusive of tags
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSEPVAARSTSIIATIGPASRSVERLKEMIKAGMNIARLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAIALDTKGPEIRTGILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWVDYPNIVRVVPVGGRIYIDDGLISLVVQKIGPEGLVTQVENGGVLGSRKGVNLPGAQVDLPGLSEQDVRDLRFGVEHGVDIVFASFVRKASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQLLSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSIS
• Sequence Similarities: Belongs to the pyruvate kinase family.

Gene Information

• Gene Name: PKLR pyruvate kinase, liver and RBC [ Homo sapiens ]
• Official Symbol: PKLR
• Synonyms: PKLR; pyruvate kinase, liver and RBC; pyruvate kinase isozymes R/L
• Gene ID: 5313
• mRNA Refseq: NM_000298
• Protein Refseq: NP_000289
• MIM: 609712
• Uniprot ID: P30613
• Chromosome Location: 1q22
• Pathway: ChREBP activates metabolic gene expression, organism-specific biosystem; Developmental Biology, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Glucose metabolism, organism-specific biosystem; Glycolysis, organism-specific biosystem
• Function: ATP binding; magnesium ion binding; nucleotide binding; potassium ion binding; pyruvate kinase activity