Recombinant Human Pituitary Tumor-transforming 1

• Cat.No.: PTTG1-487H
• Product Overview: Recombinant PTTG1 protein was expressed inE.coliand purified by using conventional chromatography techniques. MW = 24.1 kDa (222aa).

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: PTTG1, also known as Securin, is primarily involved in the regulation of sister chromatid separation during cell division. It has two identified roles; the first one is to help the transport of separase(cysteine protease) to the nucleus and the second role is to inhibit the catalytic activity of separase. It is ubiquitinated by the Anaphase Promoting Complex (APC), and then degraded by the Proteasome, releasing separase.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI.
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 0.1 M NaCl, 10% glycerol.
• Purity: > 80% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 0.5 EU per 1 μg of protein (determined by LAL method).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PTTG1 pituitary tumor-transforming 1 [ Homo sapiens ]
• Synonyms: PTTG1; pituitary tumor-transforming 1; EAP1; PTTG; HPTTG; TUTR1; MGC126883; MGC138276; securing; OTTHUMP00000160845; ESP1-associated protein 1; tumor-transforming protein 1;hPTTG; Esp1-associated protein; Tumor-transforming protein 1; pituitary tumor-transforming 1
• Gene ID: 9232
• mRNA Refseq: NM_004219
• Protein Refseq: NP_004210
• MIM: 604147
• UniProt ID: O95997
• Chromosome Location: 5q35.1
• Pathway: Cell cycle; Cell Cycle, Mito
• Function: SH3 domain binding; cysteine-type endopeptidase inhibitor activity; protein binding; transcription factor activity