Recombinant Human PIN1 293 Cell Lysate

• Cat.No.: PIN1-3179HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PIN1 peptidylprolyl cis/trans isomerase, NIMA-interacting 1 [ Homo sapiens ]
• Official Symbol: PIN1
• Synonyms: PIN1; peptidylprolyl cis/trans isomerase, NIMA-interacting 1; protein (peptidyl prolyl cis/trans isomerase) NIMA interacting 1; peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; dod; PPIase Pin1; rotamase Pin1; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; DOD; UBL5; FLJ40239; FLJ77628; MGC10717
• Gene ID: 5300
• mRNA Refseq: NM_006221
• Protein Refseq: NP_006212
• MIM: 601052
• UniProt ID: Q13526
• Chromosome Location: 19p13
• Pathway: Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; C-MYC pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; ISG15 antiviral mechanism, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Interferon Signaling, organism-specific biosystem
• Function: GTPase activating protein binding; isomerase activity; mitogen-activated protein kinase kinase binding; peptidyl-prolyl cis-trans isomerase activity; peptidyl-prolyl cis-trans isomerase activity; phosphoserine binding; phosphothreonine binding; phosphothreonine binding; protein binding