Recombinant Human PIK3R1 Protein, His-tagged

• Cat.No.: PIK3R1-4934H
• Product Overview: Recombinant full-length human PI3K (p85alpha) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Description: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.
• Source: Insect cells
• Species: Human
• Tag: His
• Molecular Mass: ~86 kDa
• Purity: > 95%
• Applications: Kinase Assay, Western Blot
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.2 μg/μL
• Storage Buffer: Recombinate protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0,1mM PMSF, 0,25mM DTT, 25% glycerol.

Gene Information

• Gene Name: PIK3R1 phosphoinositide-3-kinase regulatory subunit 1 [ Homo sapiens (human) ]
• Official Symbol: PIK3R1
• Synonyms: p85; AGM7; GRB1; IMD36; p85-ALPHA; PIK3R1; phosphoinositide-3-kinase regulatory subunit 1; phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); ptdIns-3-kinase regulatory subunit alpha
• Gene ID: 5295
• mRNA Refseq: NM_181523
• Protein Refseq: NP_852664
• MIM: 171833
• UniProt ID: P27986