Recombinant Human PIK3CG, GST-tagged

• Cat.No.: PIK3CG-55H
• Product Overview: The Recombinant Human phosphoinositide-3-kinase, catalytic, gamma polypeptide produced inSf9 insect cellsis a catalytically inactive mutant of PI3Kγ with a D946A mutation in the ATP binding site. This recombinant catalytically inactive protein can be used as a negative control in any kind of PI3Kγ kinase activity studies. Recombinant full length PI3KγDA mutant (126.3 kDa without Tag) carries a N-terminal GST-Tag which facilitates the protein's application in typical GST pull-down assays.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: This gene encodes a protein that belongs to the pi3/pi4-kinase family of proteins. The gene product is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.
• Physical Appearance: Sterile filtered liquid formualtion.
• Purity: Greater than 95.0% as determined by SDS Page.
• Formulation: 0.5mg/ml solution in 25mM Tris, pH 8.0, 50mM NaCl, 0.5mM MgCl₂, and 50% glycerol.
• Storage: PI3Kb although stable at 14°Cfor 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Full Length: Full L.

Gene Information

• Gene Name: PIK3CG phosphoinositide-3-kinase, catalytic, gamma polypeptide [ Homo sapiens ]
• Synonyms: PIK3CG; phosphoinositide-3-kinase, catalytic, gamma polypeptide; PI3K; PI3Kgamma;Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma isoform;EC2.7.1.153; PI3-kinase p110 subunit gamma; PI3Kgamma; p120-PI3K; p110-gamma; PI3Kgamma; 1-phosphatidylinositol 3-kinase; PtdIns-3-kinase subunit p110; phosphatidylinositol 3 kinase gamma, p110 gamma; phosphatidylinositol 3-kinase catalytic 110-kD gamma; phosphatidylinositol 3-kinase, catalytic, gamma polypeptide; phosphoinositide-3-kinase gamma catalytic subunit
• Gene ID: 5294
• mRNA Refseq: NM_002649
• Protein Refseq: NP_002640
• UniProt ID: P48736
• Chromosome Location: 7q
• MIM: 601232
• Pathway: Acute myeloid leukemia; Apoptosis; B cell receptor signaling pathway; Chemokine signaling pathway; Chronic myeloid leukemia; Colorectal cancer; Endometrial cancer; ErbB signaling pathway; Fc epsilon RI signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; Glioma; Inositol phosphate metabolism; Insulin signaling pathway; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Leukocyte transendothelial migration; Melanoma; Non-small cell lung cancer; Pancreatic cancer; Pathways in cancer; Phosphatidylinositol signaling system; Prostate cancer; Regulation of actin cytoskeleton; Renal cell carcinoma; Small cell lung cancer; T cell receptor signaling pathway; Toll-like receptor signaling pathway; Type II diabetes mellitus; VEGF signaling pathway; VEGF signaling pathway; mTOR signaling pathway; Hemostasis; Signaling by GPCR
• Function: 1-phosphatidylinositol-3-kinase activity; ATP binding; inositol or phosphatidylinositol kinase activity; nucleotide binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphotransferase activity, alcohol group as acceptor; protein binding; transferase activity