Recombinant Human PIGR protein(Met1-Arg638, 365Ser/Gly), His-tagged

• Cat.No.: PIGR-829H
• Product Overview: Recombinant Human PIGR (NP_002635.2)(Met 1-Arg 638, 365 Ser/Gly) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Arg638, 365Ser/Gly
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. 1. Immobilized rhhuman IgM at 2 μg/ml (100 μl/well) can bind biotinylated PIGR with a linear range of 0.94-15 ng/ml. 2. When human human IgM is immobilized at 2 μg/ml (100 μl/well), PIGR inhibits 50% binding of biotinylated PIGR (0.062 μg/ml) at the concentration range of 0.03-20 μg/ml.
• Molecular Mass: The soluble form of recombinant human PIGR consists of 630 amino acids after removal of the signal peptide and has a predicted molecular mass of 69 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 80-90 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PIGR polymeric immunoglobulin receptor [ Homo sapiens ]
• Official Symbol: PIGR
• Synonyms: PIGR; polymeric immunoglobulin receptor; poly-Ig receptor; hepatocellular carcinoma associated protein TB6; hepatocellular carcinoma-associated protein TB6; FLJ22667; MGC125361; MGC125362
• Gene ID: 5284
• mRNA Refseq: NM_002644
• Protein Refseq: NP_002635
• MIM: 173880
• UniProt ID: P01833