Recombinant Human PI4KB

• Cat.No.: PI4KB-30364TH
• Product Overview: Recombinant full length Human PI4 kinase beta with an N terminal proprietary tag; MWt 120 kDa.

Specification

• Description: Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation.
• Protein length: 801 amino acids
• Molecular Weight: 120.000kDa inclusive of tags
• Source: Baculovirus infected sf9 cells
• Tissue specificity: Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
• Biological activity: The Specific activity of PI4KB-30364TH was determined to be 32 nmol/min/mg.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.Contains 1 PI3K/PI4K domain.
• Tag: Non

Gene Information

• Gene Name: PI4KB phosphatidylinositol 4-kinase, catalytic, beta [ Homo sapiens ]
• Official Symbol: PI4KB
• Synonyms: PI4KB; phosphatidylinositol 4-kinase, catalytic, beta; PIK4CB; phosphatidylinositol 4-kinase beta; PI4K BETA; pi4K92
• Gene ID: 5298
• mRNA Refseq: NM_001198773
• Protein Refseq: NP_001185702
• MIM: 602758
• Uniprot ID: Q9UBF8
• Chromosome Location: 1q21
• Pathway: 3-phosphoinositide biosynthesis, conserved biosystem; D-myo-inositol (1,4,5)-trisphosphate biosynthesis, conserved biosystem; Inositol phosphate metabolism, organism-specific biosystem; Inositol phosphate metabolism, conserved biosystem; Inositol phosphate metabolism, PI=>
• Function: 1-phosphatidylinositol 4-kinase activity; ATP binding; kinase activity; nucleotide binding