Recombinant Human Phosphomevalonate Kinase, His-tagged

• Cat.No.: PMVK-2823H
• Product Overview: Recombinant human PMVK protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 24.1 kDa (212aa), confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Phosphomevalonate kinase, also known as PMVK, is a cytosolic enzyme. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. Induced by sterol, PMVK participates in isopentenyl diphosphate biosynthesis via the mevalonate pathway. PMVK catalyzes the conversion of mevalonate 5-phosphate into mevalonate 5-diphosphate in the fifth reaction of the cholesterol biosynthetic pathway.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAPLGGAPRL VLLFSGKRKS GKDFVTEALQ SRLGADVCAV LRLSGPLKEQ YAQEHGLNFQ RLLDTSTYKE AFRKDMIRWG EEKRQADPGF FCRKIVEGIS QPIWLVSDTR RVSDIQWFRE AYGAVTQTVR VVALEQSRQQ RGWVFTPGVD DAESECGLDN FGDFDWVIEN HGVEQRLEEQ LENLIEFIRS RL
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PMVK phosphomevalonate kinase [ Homo sapiens ]
• Synonyms: PMVK; phosphomevalonate kinase; PMK; PMKA; PMKASE; HUMPMKI; Hpmk; EC 2.7.4.2; PMKI; PMKase
• Gene ID: 10654
• mRNA Refseq: NM_006556
• Protein Refseq: NP_006547
• MIM: 607622
• UniProt ID: Q15126
• Chromosome Location: 1q22
• Pathway: Metabolic pathways; Peroxisome; Terpenoid backbone biosynthesis
• Function: ATP binding; nucleotide binding; phosphomevalonate kinase activity; protein binding; transferase activity