Recombinant Human PhosphoHistidine Phosphatase 1, His-tagged

• Cat.No.: PHPT1-800H
• Product Overview: Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 15.9 kDa (145aa) confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PHPT1 phosphohistidine phosphatase 1 [ Homo sapiens ]
• Synonyms: PHPT1; phosphohistidine phosphatase 1; PHP14; CGI-202; HSPC141; bA216L13.10; DKFZp564M173; RP11-216L13.10; 14 kDa phosphohistidine phosphatase; 1700008C22Rik; OTTHUMP00000022620; protein janus-A homolog; sex-regulated protein janus-a; phosphohistidine phosphatase 14kDa; EC 3.1.3.-
• Gene ID: 29085
• mRNA Refseq: NM_001135861
• Protein Refseq: NP_001129333
• MIM: 610167
• UniProt ID: Q9NRX4
• Chromosome Location: 9q34.3
• Pathway: Fructose and mannose metabolism; Metabolic pathways; Riboflavin metabolism; Thiamine metabolism
• Function: hydrolase activity; phosphohistidine phosphatase activity; phosphoprotein phosphatase activity