Recombinant Human phosphatidylinositol-4-phosphate 5-kinase, type I, GST-tagged

• Cat.No.: PIP5K1C-1083H
• Product Overview: Recombinant full-length human PIP5K1C was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PIP5K1C or phosphatidylinositol-4-phosphate 5-kinase 1 gamma phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1C is found at synapses and has been found to play roles in endocytosis and cell migration. PIP5K1C directly associates with β-catenin and increases β-catenin activity downstream of growth factor stimulation. PIP5K1C expression and kinase activity enhance β-catenin phosphorylation on residues that promote nuclear importation and transcriptional activity. PIP5K1C deficiency impairs neutrophil recruitment as a result of defect in adhesion. PIP5K1C regulated the adhesion through facilitating RhoA GTPase and integrin activation by chemoattractants.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 120 kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM Tris-HCL, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 0 25% glycerol.
• Specific Activity: 220 nmol/min/mg
• Purity: > 80 %
• Concentration: 0.1 ug/ul
• Sequences: Full length
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: 3-phosphoinositide biosynthesis; Arf6 trafficking events; Axon guidance; B Cell Receptor Signaling Pathway; E-cadherin signaling in the nascent adherens junction; Endocytosis; Fc gamma R-mediated phagocytosis; Focal Adhesion; Inositol phosphate metabolism; Metabolic pathways; N-cadherin signaling events; Phosphatidylinositol signaling system; Regulation of Actin Cytoskeleton; RhoA signaling pathway; Stabilization and expansion of the E-cadherin adherens junction; superpathway of inositol phosphate compounds

Gene Information

• Gene Name: PIP5K1C phosphatidylinositol-4-phosphate 5-kinase, type I, gamma [ Homo sapiens ]
• Official Symbol: PIP5K1C
• Synonyms: PIP5K1C; phosphatidylinositol-4-phosphate 5-kinase, type I, gamma; LCCS3; KIAA0589; PIPKIg_v4; PIP5Kgamma; PIP5K-GAMMA; phosphatidylinositol-4-phosphate 5-kinase type-1 gamma; PIP5K1-gamma; type I PIP kinase; diphosphoinositide kinase; ptdIns(4)P-5-kinase 1 gamma; Human homolog of mouse phosphatidylinositol-4-phosphate 5-kinase I-gamma
• Gene ID: 23396
• mRNA Refseq: NM_012398
• Protein Refseq: NP_036530
• MIM: 606102
• UniProt ID: O60331
• Chromosome Location: 19p13.3
• Function: 1-phosphatidylinositol-4-phosphate 5-kinase activity; ATP binding; kinase activity; nucleotide binding; transferase activity