Recombinant Human Phosphate Cytidylyltransferase 2, Ethanolamine, His-tagged

• Cat.No.: PCYT2-1564H
• Product Overview: Recombinant human PCYT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PCYT2 is an enzyme that catalyzes the formation of CDP-ethanolamine from CTP and phosphoethanolamine in the Kennedy pathway of phospholipid synthesis. Alternative splicing results in multiple transcript variants.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT, 0.1mM PMSF.
• Molecular Weight: 45.9 kDa (409aa), confirmed by MALDI-TOF.
• Purity: >90% by SDS-PAGE
• Concentration: 0.25 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MIRNGRGAAG GAEQPGPGGR RAVRVWCDGC YDMVHYGHSN QLRQARAMGD YLIVGVHTDE EIAKHKGPPVFTQEERYKMV QAIKWVDEVV PAAPYVTTLE TLDKYNCDFC VHGNDITLTV DGRDTYEEVK QAGRYRECKR TQGVSTTDLV GRMLLVTKAHHSSQEMSSEY REYADSFGKC PGGRNPWTGV SQFLQTSQKI IQFASGKEPQ PGETVIYVAG AFDLFHIGHV DFLEKVHRLA ERPYIIAGLHFDQEVNHYKG KNYPIMNLHE RTLSVLACRY VSEVVIGAPY AVTAELLSHF KVDLVCHGKT EIIPDRDGSD PYQEPKRRGI FRQIDSGSNLTTDLIVQRII TNRLEYEARN QKKEAKELAF LEAARQQAAQ PLGERDGDF
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Glycerophospholipid metabolism; Metabolic pathways; Phosphatidylethanolamine (PE) biosynthesis

Gene Information

• Gene Name: PCYT2 phosphate cytidylyltransferase 2, ethanolamine [ Homo sapiens ]
• Official Symbol: PCYT2
• Synonyms: PCYT2; phosphate cytidylyltransferase 2, ethanolamine; ET; ethanolamine-phosphate cytidylyltransferase; phosphorylethanolaminetransferase; CTP:phosphoethanolaminecytidylyltransferase; EC 2.7.7.14; EC 2.7.7
• Gene ID: 5833
• mRNA Refseq: NM_001184917
• Protein Refseq: NP_001171846
• MIM: 602679
• UniProt ID: Q99447
• Chromosome Location: 17q25.3
• Function: ethanolamine-phosphate cytidylyltransferase activity; nucleotidyltransferase activity; transferase activity