Recombinant Human Peroxisomal D3,D2-enoyl-CoA Isomerase, His-tagged

• Cat.No.: PECI-0605H
• Product Overview: Recombinant human PECI protein, fused to his-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 42.3 kDa (384aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: PECI, also referred to as DRS1, ACBD2 or HCA88, is a protein that localizes to the peroxisomal matrix and contains one ACB (acyl-CoA-binding) domain. This protein is expressed abundantly in liver, heart and skeletal muscle. It functions to catalyze the isomerization of both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species, playing an important role in the β-oxidation of unsaturated fatty acids.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MNRTAMRASQ KDFENSMNQV KLLKKDPGNE VKLKLYALYK QATEGPCNMP KPGVFDLINK AKWDAWNALG SLPKEAARQN YVDLVSSLSP SLESSSQVEP GTDRKSTGFE TLVVTSEDGI TKIMFNRPKK KNAINTEMYH EIMRALKAAS KDDSIITVLT GNGDYYSSGN DLTNFTDIPP GGVEEKAKNN AVLLREFVGC FIDFPKPLIA VVNGPAVGIS VTLLGLFDAV YASDRATFHT PFSHLGQSPE GCSSYTFPKI MSPAKATEML IFGKKLTAGE ACAQGLVTEV FPDSTFQKEV WTRLKAFAKL PPNALRISKE VIRKREREKL HAVNAEECNV LQGRWLSDEC TNAVVNFLSR KSKL
• Purity: > 95% by SDS – PAGE.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: PECI peroxisomal D3,D2-enoyl-CoA isomerase [ Homo sapiens ]
• Synonyms: PECI; peroxisomal D3,D2-enoyl-CoA isomerase; DRS1; ACBD2; HCA88; KIAA0536; dJ1013A10.3; peroxisomal 3,2-trans-enoyl-CoA isomerase ;DRS-1; OTTHUMP00000015976; DBI-related protein 1; dodecenoyl-CoA isomerase; D3,D2-enoyl-CoA isomerase; renal carcinoma antigen NY-REN-1; delta(3),delta(2)-enoyl-CoA isomerase; acyl-Coenzyme A binding domain containing 2; diazepam-binding inhibitor-related protein 1; hepatocellular carcinoma-associated antigen 88; EC 5.3.3.8; Peroxisomal 3,2-trans-enoyl-CoA isomerase;DRS-1
• Gene ID: 10455
• mRNA Refseq: NM_001166010
• Protein Refseq: NP_001159482
• MIM: 608024
• UniProt ID: O75521
• Chromosome Location: 6p24.3
• Pathway: Fatty acid metabolism; PeroxisomeSee PECI in MapViewer
• Function: acyl-CoA binding; binding; dodecenoyl-CoA delta-isomerase activity; isomerase activity