Recombinant Human Perilipin 2, His-tagged

• Cat.No.: PLIN2-904H
• Product Overview: Recombinant Human Perilipin 2 encoding the human adipose related protein (Met¹- His⁴³⁷) was recombined with his tag at the N-Terminus. That was expressed inE. Coli, and purified by Ni-NTA column.MW=48.1KDa.

Specification

• Cat. No.: PLIN2-904H
• Description: Adipocyte differentiation-related protein is associated with the globule surface membrane material. This protein is a major constituent of the globule surface. Increase in mRNA levels is one of the earliest indications of adipocyte differentiation.
• Source: E. Coli.
• Sequence: Human adipose differentiation related protein (Met¹-His⁴³⁷)1MASVAVDPQP SVVTRVVNLP LVSSTYDLMS SAYLSTKDQY PYLKSVCEMA ENGVKTITSV AMTSALPIIQ KLEPQIAVAN TYACKGLDRI EERLPILNQP STQIVANAKG AVTGAKDAVT TTVTGAKDSV ASTITGVMDK TKGAVTGSVE KTKSVVSGSI NTVLGSRMMQ LVSSGVENAL TKSELLVEQY LPLTEEELEK EAKKVEGFDL VQKPSYYVRL GSLSTKLHSR AYQQALSRVK EAKQKSQQTI SQLHSTVHLI EFARKNVYSA NQKIQDAQDK LYLSWVEWKR SIGYDDTDES HCAEHIESRT LAIARNLTQQ LQTTCHTLLS NIQGVPQNIQ DQAKHMGVMA GDIYSVFRNA ASFKEVSDSL LTSSKGQLQK MKESLDDVMD YLVNNTPLNW LVGPFYPQLT ESQNAQDQGA EMDKSSQETQ RSEHKTH 437.
• Purity: >95% in SDS gel.
• Formulation: Lyophilized 100 μg human Perilipin 2 in 50 μl of TBS (20 mM Tris, 50mM NaCl, pH8.0). Carry free.
• Application: Cell biology, ELISA.
• Reconstitution: Add 500 μl deionized water to the vial to prepare a working stock solution at 200μg/mL. Allow to set at least 30 minutes at 4°C, mix well.
• Storage: Store lyophilized protein at -20°C or -70°C. Lyophilized protein is stable for up to 6 months from date of receipt at -70°C. Upon reconstitution, this protein can be stored at for a few weeks or at -70°C in a manual defrost freezer for long term storage (six months). Aliquot reconstituted protein to avoid repeated freezing / thawing cycles.
• Protein length: Met1-His437

Gene Information

• Gene Name: PLIN2 perilipin 2 [ Homo sapiens ]
• Synonyms: ADFP; ADRP; PLIN2; adipose differentiation-related protein; adipophilin; MGC10598; Adipose differentiation-related protein; Adipophilin; Perilipin 2
• Gene ID: 123
• mRNA Refseq: NM_001122
• Protein Refseq: NP_001113
• MIM: 103195
• UniProt ID: Q99541
• Chromosome Location: 9p22.1
• Pathway: Metabolism of lipids and lipoproteins
• Function: protein binding

Citation

Aromatic Functionality of Target Proteins Influences Monomer Selection for Creating Artificial Antibodies on Plasmonic Biosensors

Journal: ACS applied materials & interfaces    PubMed ID: 27935290    Data: 2017/4/11

Authors: Rong Hu, Jingyi Luan, Jeremiah J. Morrissey

Article Snippet:Trimethoxyphenylsilane (TMPhS, 97%), trimethoxypropylsilane (TMPS, 97%), (3-aminopropyl) trimethoxysilane (APTMS, 99%), p -aminothiophenol (p-ATP), glutaraldehyde (Grade I, 25% in water), cetyltrimethylammonium bromide (CTAB), lysozyme, human serum albumin, and hemoglobin (all lyophilized powders) were purchased from Sigma-Aldrich (St. Louis).Trimethoxyphenylsilane (TMPhS, 97%), trimethoxypropylsilane (TMPS, 97%), (3-aminopropyl) trimethoxysilane (APTMS, 99%), p -aminothiophenol (p-ATP), glutaraldehyde (Grade I, 25% in water), cetyltrimethylammonium bromide (CTAB), lysozyme, human serum albumin, and hemoglobin (all lyophilized powders) were purchased from Sigma-Aldrich (St. Louis).. Recombinant human Perilipin-2 (PLIN2) (liquid in 20 mM Tris-HCl buffer, pH 8.0) was purchased from Creative BioMart (Shirley, NY).. Gold Nanorod Synthesis Gold nanorods (AuNRs) were synthesized by using a seed-mediated method.Gold Nanorod Synthesis Gold nanorods (AuNRs) were synthesized by using a seed-mediated method.

(A) Sensitivity of MIP-AuNR nanosensor expressed by the shift of LSPR wavelength when detecting PLIN2 at different concentrations with polymers of different compositions. (B) Specificity of the PLIN2-imprinted artificial antibody compared with interference protein (HSA or BSA) at distinct concentrations with polymers of different compositions. (C) Sensitivity of MIP-AuNR nanosensor expressed by the shift of LSPR wavelength when detecting hemoglobin at different concentrations with polymers of different compositions. (D) LSPR shift of interference proteins (HSA or BSA) on hemoglobin-imprinted plasmonic artificial antibody with polymers of different compositions.

Publication :

Aromatic Functionality of Target Proteins Influences Monomer Selection for Creating Artificial Antibodies on Plasmonic Biosensors. (2016)

Reviews

01/15/2022

Operation is simple and does not require complex operation steps.

10/06/2021

Quality I use is very good and I have recommended this product to my colleagues and friends.

01/24/2021

Sensitivity in Western blot is very high and low concentrations of the target protein can be detected.

Q&As

How are the interactions between PLIN2 and other proteins regulated?

The interaction between PLIN2 and other proteins is regulated by the formation of complexes or the regulation of signal transduction pathways. These interactions can be influenced by a variety of factors, such as nutrients, hormones, and inflammatory factors.

What is the relationship between PLIN2 and cardiovascular disease?

Some studies have suggested that the expression level of PLIN2 may be associated with the occurrence and progression of cardiovascular disease. High levels of PLIN2 may be associated with an increased risk of cardiovascular diseases such as atherosclerosis, myocardial hypertrophy, and heart failure.

Can the function of PLIN2 be studied by gene knockout or knockdown techniques?

The function of PLIN2 can be studied through gene knockout or knockdown techniques, such as using techniques such as CRISPR-Cas9 or RNAi. These techniques can create cellular models of PLIN2 deletion or reduction to observe its effects on fat storage and breakdown, among other things.

What is the role of PLIN2 in obesity and diabetes?

PLIN2 plays an important role in obesity and diabetes. It may influence the development of obesity and diabetes by regulating adipocyte differentiation, insulin sensitivity, and lipid metabolism.

How can the function of PLIN2 be studied by overexpression techniques?

PLIN2 can be studied through overexpression techniques, such as the use of gene transduction or viral transduction. These techniques can increase the expression level of PLIN2 to observe its effects on fat storage and breakdown, among other things.

How does the expression level of PLIN2 relate to the occurrence and progression of the disease?

Some studies have shown that PLIN2 expression levels may be associated with obesity, diabetes, and cardiovascular disease, among others. High levels of PLIN2 may be associated with increased susceptibility to disease, while low levels of PLIN2 may be associated with decreased susceptibility to disease.