Recombinant Human PELI1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PELI1-5917H
• Product Overview: PELI1 MS Standard C13 and N15-labeled recombinant protein (NP_065702) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation.
• Molecular Mass: 46.3 kDa
• AA Sequence: MFSPDQENHPSKAPVKYGELIVLGYNGSLPNGDRGRRKSRFALFKRPKANGVKPSTVHIACTPQAAKAISNKDQHSISYTLSRAQTVVVEYTHDSNTDMFQIGRSTESPIDFVVTDTVPGSQSNSDTQSVQSTISRFACRIICERNPPFTARIYAAGFDSSKNIFLGEKAAKWKTSDGQMDGLTTNGVLVMHPRNGFTEDSKPGIWREISVCGNVFSLRETRSAQQRGKMVEIETNQLQDGSLIDLCGATLLWRTAEGLSHTPTVKHLEALRQEINAARPQCPVGFNTLAFPSMKRKDVVDEKQPWVYLNCGHVHGYHNWGNKEERDGKDRECPMCRSVGPYVPLWLGCEAGFYVDAGPPTHAFSPCGHVCSEKTTAYWSQIPLPHGTHTFHAACPFCAHQLAGEQGYIRLIFQGPLDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PELI1 pellino E3 ubiquitin protein ligase 1 [ Homo sapiens (human) ]
• Official Symbol: PELI1
• Synonyms: PELI1; pellino E3 ubiquitin protein ligase 1; E3 ubiquitin-protein ligase pellino homolog 1; RING-type E3 ubiquitin transferase pellino homolog 1; pellino homolog 1; pellino-1; pellino-related intracellular-signaling molecule; protein pellino homolog 1; EC 2.3.2.27
• Gene ID: 57162
• mRNA Refseq: NM_020651
• Protein Refseq: NP_065702
• MIM: 614797
• UniProt ID: Q96FA3