Recombinant Human PDLIM1, His-tagged

Cat.No. : PDLIM1-5031H
Product Overview : Recombinant human PDLIM1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
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Species : Human
Source : E.coli
Tag : His
Description : PDLIM1 is a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17.
Form : Liquid. In 20mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT, 2mM EDTA
Molecular Mass : 38.7 kDa (354aa) confirmed by MALDI-TOF
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSEFMTTQQ IDLQGPGPWG FRLVGGKDFE QPLAISRVTP GSKAALANLC IGDVITAIDG ENTSNMTHLE AQNRIKGCTD NLTLTVARSE HKVWSPLVTE EGKRHPYKMN LASEPQEVLH IGSAHNRSAM PFTASPASST TARVITNQYN NPAGLYSSEN ISNFNNALES KTAASGVEAN SRPLDHAQPP SSLVIDKESE VYKMLQEKQE LNEPPKQSTS FLVLQEILES EEKGDPNKPS GFRSVKAPVT KVAASIGNAQ KLPMCDKCGT GIVGVFVKLR DRHRHPECYV CTDCGTNLKQ KGHFFVEDQI YCEKHARERV TPPEGYEVVT VFPK
Purity : >85% by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name PDLIM1 PDZ and LIM domain 1 [ Homo sapiens ]
Official Symbol PDLIM1
Synonyms PDLIM1; PDZ and LIM domain 1; CLIM1, PDZ and LIM domain 1 (elfin); PDZ and LIM domain protein 1; carboxyl terminal LIM domain protein 1; CLP 36; CLP36; elfin; hCLIM1; enigma; LIM domain protein CLP-36; C-terminal LIM domain protein 1; carboxy terminal LIM domain protein 1; CLIM1; CLP-36;
Gene ID 9124
mRNA Refseq NM_020992
Protein Refseq NP_066272
MIM 605900
UniProt ID O00151
Chromosome Location 10q23.1
Function metal ion binding; transcription coactivator activity; zinc ion binding;

Not For Human Consumption!

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