Recombinant Human PDK1 protein(Ser29-Ala436), His-tagged

• Cat.No.: PDK1-904H
• Product Overview: Recombinant Human PDK1 (NP_002601.1) (Ser 29-Ala 436) was expressed in Insect Cells, with a polyhistidine tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His
• Protein length: 29-436 a.a.
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human PDK1 consists of 427 amino acids and has a calculated molecular mass of 48.6 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDK1 pyruvate dehydrogenase kinase, isozyme 1 [ Homo sapiens ]
• Official Symbol: PDK1
• Synonyms: PDK1; pyruvate dehydrogenase kinase, isozyme 1; pyruvate dehydrogenase kinase, isoenzyme 1; mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
• Gene ID: 5163
• mRNA Refseq: NM_002610
• Protein Refseq: NP_002601
• MIM: 602524
• UniProt ID: Q15118