Recombinant Human PDHA1, His-GST tagged

• Cat.No.: PDHA1-245H
• Product Overview: Recombinant Human PDHA1 (P08559-1) (Phe30-Ser390), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• ProteinLength: 30-390 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20mM Tris, 500mM Nacl, 3mM DTT, 10% gly, pH 8.0.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant human PDHA1/GST chimera consists of 598 amino acids and has a calculated molecular mass of 68 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: PDHA1 pyruvate dehydrogenase (lipoamide) alpha 1 [ Homo sapiens ]
• Official Symbol: PDHA1
• Gene ID: 5160
• mRNA Refseq: NM_000284
• Protein Refseq: NP_000275
• MIM: 300502
• UniProt ID: P08559
• Chromosome Location: Xp22.1