Recombinant Human PDHA1 protein(Phe30-Ser390)

• Cat.No.: PDHA1-2372H
• Product Overview: Recombinant Human PDHA1 (P08559-1) (Phe30-Ser390) was expressed in Insect Cells, fused with two additional amino acids (Gly & Pro) at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: Non
• Protein Length: Phe30-Ser390
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human PDHA1 consists of 363 amino acids and has a calculated molecular mass of 40.4 kDa. The recombinant protein migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PDHA1 pyruvate dehydrogenase (lipoamide) alpha 1 [ Homo sapiens ]
• Official Symbol: PDHA1
• Synonyms: PDHA1; pyruvate dehydrogenase (lipoamide) alpha 1; PDHA; pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; PHE1A; PDHCE1A
• Gene ID: 5160
• mRNA Refseq: NM_000284
• Protein Refseq: NP_000275
• MIM: 300502
• UniProt ID: P08559