Recombinant Human PDE7A protein, His-tagged

• Cat.No.: PDE7A-2928H
• Product Overview: Recombinant Human PDE7A protein(1-55 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 1-55 aa
• AA Sequence: MGITLIWCLALVLIKWITSKRRGAISYDSSDQTALYIRMLGDVRVRSRAGFESER
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: PDE7A phosphodiesterase 7A [ Homo sapiens ]
• Official Symbol: PDE7A
• Synonyms: PDE7A; phosphodiesterase 7A; high affinity cAMP-specific 3,5-cyclic phosphodiesterase 7A; HCP1; TM22; phosphodiesterase isozyme 7; PDE7
• Gene ID: 5150
• mRNA Refseq: NM_001242318
• Protein Refseq: NP_001229247
• MIM: 171885
• UniProt ID: Q13946