Recombinant Human PDE1B Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: PDE1B-2292H
• Product Overview: PDE1B MS Standard C13 and N15-labeled recombinant protein (NP_000915) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
• Molecular Mass: 61.4 kDa
• AA Sequence: MELSPRSPPEMLEESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEINIEELKKNLEYTASLLEAVYIDETRQILDTEDELQELRSDAVPSEVRDWLASTFTQQARAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTSVGPTYSTAVLNCLKNLDLWCFDVFSLNQAADDHALRTIVFELLTRHNLISRFKIPTVFLMSFLDALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEIELLAIIFAAAIHDYEHTGTTNSFHIQTKSECAIVYNDRSVLENHHISSVFRLMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQQLERIDKPKALSLLLHAADISHPTKQWLVHSRWTKALMEEFFRQGDKEAELGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLADEDSKSKNQPSFQWRQPSLDVEVGDPNPDVVSFRSTWVKRIQENKQKWKERAASGITNQMSIDELSPCEEEAPPSPAEDEHNQNGNLDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: PDE1B phosphodiesterase 1B [ Homo sapiens (human) ]
• Official Symbol: PDE1B
• Synonyms: PDE1B; phosphodiesterase 1B, calmodulin-dependent; PDES1B; calcium/calmodulin-dependent 3,5-cyclic nucleotide phosphodiesterase 1B; cam-PDE 1B; 63 kDa Cam-PDE; calmodulin-stimulated phosphodiesterase PDE1B1; calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase; presumed 63kDa form of the type 1 cyclic nucleotide phosphodiesterase family known as PDE1B; PDE1B1
• Gene ID: 5153
• mRNA Refseq: NM_000924
• Protein Refseq: NP_000915
• MIM: 171891
• UniProt ID: Q01064