Recombinant Human PDCD6IP protein, His & GST-tagged

• Cat.No.: PDCD6IP-6715H
• Product Overview: Recombinant Human PDCD6IP aa. (Glu174~Val383 (Accession # Q8WUM4)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: Glu174~Val383
• Description: This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 53.8kDa.
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: PDCD6IP programmed cell death 6 interacting protein [ Homo sapiens (human) ]
• Official Symbol: PDCD6IP
• Synonyms: AIP1; ALIX; HP95; DRIP4; ALG-2 interacting protein 1; ALG-2-interacting protein X; PDCD6-interacting protein; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; programmed cell death 6-interacting protein
• Gene ID: 10015
• mRNA Refseq: NM_001162429.2
• Protein Refseq: NP_001155901.1
• UniProt ID: Q8WUM4