Recombinant Human PCK1, His-tagged

• Cat.No.: PCK1-29962TH
• Product Overview: Recombinant full length protein of Human PCK1 fused to His tag at N-terminus, 646 amino acids with a predicted MWt 71.7kDa.

Specification

• Description: This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.
• Protein length: 622 amino acids
• Conjugation: HIS
• Molecular Weight: 71.700kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Major sites of expression are liver, kidney and adipocytes.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 10% Glycerol, 0.02% DTT, 0.58% Sodium chloride
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMPPQLQ NGLNLSAKVV QGSLDSLPQA VREFLENNAE LCQPDHIHIC DGSEEENGRL LGQMEEEGIL RRLKKYDNCW LALTDPRDVA RIESKTVIVT QEQRDTVPIP KTGLSQLGRW MSEEDFEKAF NARFPGCMKG RTMYVIPFSM GPLGSPLSKI GIELTDSPYV VASMRIMTRM GTPVLEALGD GEFVKCLHSV GCPLPLQKPL VNNWPCNPEL TLIAHLPDRR EIISFGSGYG GNSLLGKKCF ALRMASRLAK EEGWLAEHML VLGITNPEGE KKYLAAAFPS ACGKTNLAMM NPSLPGWKVE CVGDDIAWMK FDAQGHLRAI NPENGFFGVA PGTSVKTNPN AIKTIQKNTI FTNVAETSDG GVYWEGIDEP LASGVTITSW KNKEWSSEDG EPCAHPNSRF CTPASQCPII DAAWESPEGV PIEGIIFGGR RPAGVPLVYE ALSWQHGVFV GAAMRSEATA AAEHKGKIIM HDPFAMRPFF GYNFGKYLAH WLSMAQHPAA KLPKIFHVNW FRKDKEGKFL WPGFGENSRV LEWMFNRIDG KASTKLTPIG YIPKEDALNL KGLGHINMME LFSISKEFWE KEVEDIEKYL EDQVNADLPC EIEREILALK QRISQM
• Sequence Similarities: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Gene Information

• Gene Name: PCK1 phosphoenolpyruvate carboxykinase 1 (soluble) [ Homo sapiens ]
• Official Symbol: PCK1
• Synonyms: PCK1; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEPCK C
• Gene ID: 5105
• mRNA Refseq: NM_002591
• Protein Refseq: NP_002582
• MIM: 614168
• Uniprot ID: P35558
• Chromosome Location: 20q13.31
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Adipogenesis, organism-specific biosystem; Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem
• Function: GDP binding; GTP binding; carboxylic acid binding; lyase activity; magnesium ion binding