Recombinant Human PCBP2

• Cat.No.: PCBP2-29961TH
• Product Overview: Recombinant full length Human PCBP2/hnRNP E2 with a N terminal proprietary tag; Predicted MWt 65.93 kDa.

Specification

• Species: Human
• Source: Wheat Germ
• Tag: Non
• ProteinLength: 365 amino acids
• Description: The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
• Molecular Weight: 65.930kDa inclusive of tags
• Tissue specificity: Detected in all tissues examined.
• Form: Liquid
• Purity: Proprietary Purification
• Storage buffer: pH: 8.00Constituents:0.79% Tris HCl, 0.3% Glutathione
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequences of amino acids: MDTGVIEGGLNVTLTIRLLMHGKEVGSIIGKKGESVKKMR EESGARINISEGNCPERIITLAGPTNAIFKAFAMIIDKLE EDISSSMTNSTAASRPPVTLRLVVPASQCGSLIGKGGCKI KEIRESTGAQVQVAGDMLPNSTERAITIAGIPQSIIECVK QICVVMLESPPKGVTIPYRPKPSSSPVIFAGGQDRYSTGS DSASFPHTTPSMCLNPDLEGPPLEAYTIQGQYAIPQPDLT KLHQLAMQQSHFPMTHGNTGFSGIESSSPEVKGYWAGLDA SAQTTSHELTIPNDLIGCIIGRQGAKINEIRQMSGAQIKI ANPVEGSTDRQVTITGSAASISLAQYLINVRLSSETGGMG SS
• Sequence Similarities: Contains 3 KH domains.

Gene Information

• Gene Name: PCBP2 poly(rC) binding protein 2 [ Homo sapiens ]
• Official Symbol: PCBP2
• Synonyms: PCBP2; poly(rC) binding protein 2; poly(rC)-binding protein 2; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; HNRPE2
• Gene ID: 5094
• mRNA Refseq: NM_001098620
• Protein Refseq: NP_001092090
• MIM: 601210
• Uniprot ID: Q15366
• Chromosome Location: 12q13.12-q13.13
• Pathway: Formation and Maturation of mRNA Transcript, organism-specific biosystem; Gene Expression, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Negative regulators of RIG-I/MDA5 signaling, organism-specific biosystem
• Function: DNA binding; RNA binding; RNA binding; enzyme binding; protein binding