Recombinant Human PAX3 293 Cell Lysate

• Cat.No.: PAX3-3418HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for paired box 3 (PAX3), transcript variant PAX3D is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PAX3 paired box 3 [ Homo sapiens ]
• Official Symbol: PAX3
• Synonyms: PAX3; paired box 3; paired box gene 3 (Waardenburg syndrome 1) , Waardenburg syndrome 1 , WS1; paired box protein Pax-3; HUP2; paired domain gene 3; paired domain gene HuP2; paired box homeotic gene 3; WS1; WS3; CDHS; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778
• Gene ID: 5077
• mRNA Refseq: NM_000438
• Protein Refseq: NP_000429
• MIM: 606597
• UniProt ID: P23760
• Chromosome Location: 2q36.1
• Pathway: Regulation of retinoblastoma protein, organism-specific biosystem; Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: chromatin binding; protein binding; sequence-specific DNA binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific distal enhancer binding RNA polymerase II transcription factor activity