Recombinant Human PARP1 Protein

• Cat.No.: PARP1-59H
• Product Overview: Recombinant Human PARP1 Protein without tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
• Unit Definition: One unit of PARP incorporates 10 femtomoles of NAD onto immobilized histones in 30 minutes. High specific activity PARP is provided at 10 units/μL.
• Applications: Identification of inhibitors of PARP1 activity
  Quantitation of DNA Damage
  Investigation of PARP1 inactivation during apoptosis
  Western Blot Analysis
• Stability: The enzyme retains over 66 % of its activity after 24 hr at 37 centigrade.
• Storage: Store at -20 centigrade in a manual defrost freezer. For long-term storage, freeze in working aliquots at -80 centigrade. Avoid freeze-thaw cycles.
• Concentration: 10 units/μL
• Storage Buffer: 20 mM Tris-Cl (pH 8.0), 200 mM NaCl, 1 mM DTT, 0.1% Triton X-100, 50% glycerol, and 0.1 mg/ml BSA.

Gene Information

• Gene Name: PARP1 poly(ADP-ribose) polymerase 1 [ Homo sapiens (human) ]
• Official Symbol: PARP1
• Synonyms: PARP1; poly(ADP-ribose) polymerase 1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; poly [ADP-ribose] polymerase 1; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase); ADP-ribosyltransferase NAD(+); ADP-ribosyltransferase diphtheria toxin-like 1; DNA ADP-ribosyltransferase PARP1; NAD(+) ADP-ribosyltransferase 1; poly (ADP-ribose) polymerase family, member 1; poly(ADP-ribose) synthetase; poly(ADP-ribosyl)transferase; poly[ADP-ribose] synthase 1; protein poly-ADP-ribosyltransferase PARP1; EC 2.4.2.30
• Gene ID: 142
• mRNA Refseq: NM_001618
• Protein Refseq: NP_001609
• MIM: 173870
• UniProt ID: P09874