Recombinant Human PARD6B, His-tagged

• Cat.No.: PARD6B-7058H
• Product Overview: Recombinant human PARD6B protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-372aa
• Description: Partitioning defective 6 homolog beta, also known as PARD6B, is a member of the PAR6 family and encodes a protein with a PSD95/Discs-large/ZO1 (PDZ) domain, an OPR domain and a semi-Cdc42/Rac interactive binding (CRIB) domain. Cellular asymmetry is critical for the development of multicellular organisms. PARD (partitioning-defective) proteins play important roles in asymmetric cell division and polarized growth, whereas Cdc42 and Rac mediate establishment of cell growth and polarity and contribute to oncogenic transformation by Ras. It is expressed in pancreas and in both adult and fetal kidney, and is weakly expressed in placenta and lung.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2M Urea, 20% glycerol, 0.2M NaCl
• Molecular Mass: 43.6 kDa (395aa)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNRSHRH GAGSGCLGTM EVKSKFGAEF RRFSLERSKP GKFEEFYGLL QHVHKIPNVD VLVGYADIHG DLLPINNDDN YHKAVSTANP LLRIFIQKKE EADYSAFGTD TLIKKKNVLT NVLRPDNHRK KPHIVISMPQ DFRPVSSIID VDILPETHRR VRLYKYGTEK PLGFYIRDGS SVRVTPHGLE KVPGIFISRL VPGGLAQSTG LLAVNDEVLE VNGIEVSGKS LDQVTDMMIA NSRNLIITVR PANQRNNVVR NSRTSGSSGQ STDNSLLGYP QQIEPSFEPE DEDSEEDDII IEDNGVPQQI PKAVPNTESL ESLTQIELSF ESGQNGFIPS NEVSLAAIAS SSNTEFETHA PDQKLLEEDG TIITL
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml

Gene Information

• Gene Name: PARD6B par-6 partitioning defective 6 homolog beta (C. elegans) [ Homo sapiens ]
• Official Symbol: PARD6B
• Synonyms: PARD6B; par-6 partitioning defective 6 homolog beta (C. elegans); par 6 (partitioning defective 6, C.elegans) homolog beta; partitioning defective 6 homolog beta; PAR 6B; PAR-6 beta; PAR6B
• Gene ID: 84612
• mRNA Refseq: NM_032521
• Protein Refseq: NP_115910
• MIM: 608975
• UniProt ID: Q9BYG5
• Chromosome Location: 20q13.13
• Pathway: Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Tight junction, organism-specific biosystem; Tight junction, conserved biosystem
• Function: protein binding