Recombinant Human PAK3, GST-His

• Cat.No.: PAK3-722H
• Product Overview: RecombinantHuman PAK3, full length, amino acids M1-R544, N-terminally fused to GST-HIS6-Thrombin cleavage site, was expressed inSf9insect cells. MW = 90,588 Da.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-544 a.a.
• Description: PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets.
• Purification: One-step affinity purification using GSH-agarose.
• Product Identity: PAK3 was confirmed as PAK3 by by mass spectroscopy LC-ESI-MS/MS (Protagen AG,Germany).
• Storage Buffer: 50 mM HEPES-NaOH, pH 7.5; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.
• Concentration: 0.360 μg/μl (Bradford method using BSA [Sigma, cat# A-7638, Lot 79H7641] as standard protein).
• Specific Activity: 79 pmol/μg×min.
• Storage: -80°C. Avoid repeated freeze-thaw cycles!
• Full Length: Full L.

Gene Information

• Gene Name: PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 [ Homo sapiens ]
• Synonyms: PAK3; p21 protein (Cdc42/Rac)-activated kinase 3; bPAK; MRX30; MRX47; OPHN3; hPAK3; CDKN1A; PAK3beta; p21-activated kinase 3; beta-PAK; oligophrenin-3; OTTHUMP00000023856; p21 (CDKN1A)-activated kinase 3; serine/threonine-protein kinase PAK 3; EC 2.7.11.1
• Gene ID: 5063
• mRNA Refseq: NM_001128166
• Protein Refseq: NP_001121638
• MIM: 300142
• UniProt ID: O75914
• Chromosome Location: Xq23
• Pathway: Axon guidance; ErbB signaling pathway; Focal adhesion; Regulation of actin cytoskeleton; Renal cell carcinoma; T cell receptor signaling pathway; Axon guidance
• Function: ATP binding; collagen binding; nucleotide binding; protein serine/threonine kinase activity; transferase activity