Recombinant Human PAK1, GST-tagged, Active

• Cat.No.: PAK1-378H
• Product Overview: Recombinant full-length human PAK1 was expressed bybaculovirusinSf9 insect cellsusing an N-terminal GST tag. MW=96 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PAK1 is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, CDC42 and RAC. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3. PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PAK1 p21 protein (Cdc42/Rac)-activated kinase 1 [ Homo sapiens ]
• Synonyms: PAK1; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; PAKalpha; MGC130000; MGC130001; STE20 homolog, yeast; p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); Serine/threonine-protein kinase PAK 1; EC 2.7.11.1; PAK-1; p65-PAK; Alpha-PAK
• Gene ID: 5058
• mRNA Refseq: NM_001128620
• Protein Refseq: NP_001122092
• MIM: 602590
• UniProt ID: Q13153
• Chromosome Location: 11q13-q14
• Pathway: Axon guidance; Chemokine signaling pathway; Epithelial cell signaling in Helicobacter pylori infection; ErbB signaling pathway; Fc gamma R-mediated phagocytosis; Focal adhesion; MAPK signaling pathway; Natural killer cell mediated cytotoxicity; Regulation of actin cytoskeleton; Renal cell carcinoma; T cell receptor signaling pathway; Axon guidance; Signaling in Immune system
• Function: ATP binding; collagen binding; nucleotide binding; protein serine/threonine kinase activity; transferase activity