Recombinant Human PADI1 Protein, His-tagged

• Cat.No.: PADI1-011H
• Product Overview: Recombinant Human PADI1 protein (2-663) with a N-terminal His-tag was expressed in E. coli

Specification

• Description: This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type I enzyme is involved in the late stages of epidermal differentiation, where it deiminates filaggrin and keratin K1, which maintains hydration of the stratum corneum, and hence the cutaneous barrier function. This enzyme may also play a role in hair follicle formation. This gene exists in a cluster with four other paralogous genes.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 76.4 kDa
• Protein length: 2-663
• Purity: ≥95% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of NH 4 + per minute at 37 centigrade in 50 mM HEPES, pH 7.7, containing 10 mM calcium chloride, 5 mM DTT, and 2 mM N-Benzoyl-L-Arginine Ethyl Ester (BAEE).
• Stability: ≥ 1 year
• Storage: -80 centigrade
• Storage Buffer: 50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride, 1mM DTT, and 20% glycerol
• Shipping: Dry ice in continental US, may vary elsewhere.

Gene Information

• Gene Name: PADI1 peptidyl arginine deiminase 1 [ Homo sapiens (human) ]
• Official Symbol: PADI1
• Synonyms: PADI1; peptidyl arginine deiminase 1; PDI; PAD1; PDI1; HPAD10; protein-arginine deiminase type-1; hPAD-colony 10; peptidyl arginine deiminase, type I; peptidylarginine deiminase I; protein-arginine deiminase type I; EC 3.5.3.15
• Gene ID: 29943
• mRNA Refseq: NM_013358
• Protein Refseq: NP_037490
• MIM: 607934
• UniProt ID: Q9ULC6