Recombinant Human P4HB, MYC/DDK-tagged

• Cat.No.: P4HB-116H
• Product Overview: Recombinant Human P4HB fused with C-terminal MYC/DDK, was expressed in HEK293 Cells.

Specification

• Species: Human
• Source: HEK293
• Tag: DDK&Myc
• Description: This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
• Molecular Mass: 55.2 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Storage: Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: P4HB prolyl 4-hydroxylase, beta polypeptide [ Homo sapiens (human) ]
• Official Symbol: P4HB
• Synonyms: P4HB; DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; ERBA2L; P4Hbeta; prolyl 4-hydroxylase, beta polypeptide; protein disulfide-isomerase; p55; protocollagen hydroxylase; prolyl 4-hydroxylase subunit beta; collagen prolyl 4-hydroxylase beta; thyroid hormone-binding protein p55; glutathione-insulin transhydrogenase; cellular thyroid hormone-binding protein; protein disulfide isomerase-associated 1; protein disulfide isomerase/oxidoreductase; protein disulfide isomerase family A, member 1; procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide; EC 5.3.4.1
• Gene ID: 5034
• mRNA Refseq: NM_000918
• Protein Refseq: NP_000909
• MIM: 176790
• UniProt ID: P07237
• Chromosome Location: 17q25
• Pathway: Chylomicron-mediated lipid transport; Collagen biosynthesis and modifying enzymes; Collagen formation
• Function: electron carrier activity; procollagen-proline 4-dioxygenase activity; protein binding