Recombinant Human P4HB, His-tagged

• Cat.No.: P4HB-27008TH
• Product Overview: Recombinant Full-length Human P4HB with an N-terminal 6xHis-tag is a single, non-glycosylated, polypeptide chain containing 508 amino acids and having a molecular mass of 57 kDa.

Specification

• Description: This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
• Conjugation: HIS
• Source: E. coli
• Biological activity: Reductase Activity:1.0 x 10-3 OD650nm/ min-2. By measuring the turbidity increase at 650 nm due to insulin reduction (Holmgren, A. (1979) J. Biol. Chem. 254, 9627–9632). The activity is expressed as the ratio of the slope of a linear part of the turbidity
• Form: Lyophilised:Reconstitutein sterile 18Mohm/cmwater at not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 50mM Potassium phosphate, pH 7.5
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Ala.
• Sequence Similarities: Belongs to the protein disulfide isomerase family.Contains 2 thioredoxin domains.

Gene Information

• Gene Name: P4HB prolyl 4-hydroxylase, beta polypeptide [ Homo sapiens ]
• Official Symbol: P4HB
• Synonyms: P4HB; prolyl 4-hydroxylase, beta polypeptide; ERBA2L, PO4DB, procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), beta polypeptide , procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), beta polypeptide (prote
• Gene ID: 5034
• mRNA Refseq: NM_000918
• Protein Refseq: NP_000909
• MIM: 176790
• Uniprot ID: P07237
• Chromosome Location: 17q25
• Pathway: Chylomicron-mediated lipid transport, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem
• Function: electron carrier activity; isomerase activity; procollagen-proline 4-dioxygenase activity; protein binding; protein disulfide isomerase activity