Recombinant Human P2RX7 293 Cell Lysate

• Cat.No.: P2RX7-3495HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: P2RX7 purinergic receptor P2X, ligand-gated ion channel, 7 [ Homo sapiens ]
• Official Symbol: P2RX7
• Synonyms: P2RX7; purinergic receptor P2X, ligand-gated ion channel, 7; P2X purinoceptor 7; MGC20089; P2X7; ATP receptor; P2Z receptor; P2X7 receptor; purinergic receptor P2X7 variant A
• Gene ID: 5027
• mRNA Refseq: NM_002562
• Protein Refseq: NP_002553
• MIM: 602566
• UniProt ID: Q99572
• Chromosome Location: 12q24
• Pathway: Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Immune System, organism-specific biosystem; Inflammasomes, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem
• Function: ATP binding; ATP binding; extracellular ATP-gated cation channel activity; ion channel activity; lipopolysaccharide binding; protein homodimerization activity; purinergic nucleotide receptor activity; receptor activity; receptor binding

Q&As

I'm contacting you with regard to command P2RX7 Protein to carry on Western Blot experiments on human celles (Jeg-3...) . Which one could be a better candidate in your catalog, if you can recommand me?

Based on your requests, we would like to recommend you choose our cell lysate which contains full-length P2RX7 protein. It is suitable for Western Blot assay.