Name: Recombinant Human OXCT2 293 Cell Lysate
SKU: OXCT2-3507HCL

Species :	Human
Source :	HEK293
Tag :	Non
Description :	Antigen standard for 3-oxoacid CoA transferase 2 (OXCT2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Components :	This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size :	0.1 mg
Storage Instruction :	Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications :	ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Name	OXCT2 3-oxoacid CoA transferase 2 [ Homo sapiens ]
Official Symbol	OXCT2
Synonyms	OXCT2; 3-oxoacid CoA transferase 2; succinyl-CoA:3-ketoacid-coenzyme A transferase 2, mitochondrial; FKSG25; FLJ00030; SCOT T; 3-oxoacid-CoA transferase 2A; testis-specific succinyl CoA:3-oxoacid CoA-transferase; testis-specific succinyl-CoA:3-oxoacid CoA-transferase; SCOTT;
Gene ID	64064
mRNA Refseq	NM_022120
Protein Refseq	NP_071403
MIM	610289
UniProt ID	Q9BYC2
Chromosome Location	1p34
Pathway	Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; Synthesis and degradation of ketone bodies, organism-specific biosystem; Synthesis and degradation of ketone bodies, conserved biosystem; Valine, leucine and isoleucine degradation, organism-specific biosystem; Valine, leucine and isoleucine degradation, conserved biosystem;
Function	3-oxoacid CoA-transferase activity; transferase activity;