Recombinant Human OTUD1 Protein, His-tagged

• Cat.No.: OTUD1-2534H
• Product Overview: Recombinant Human OTUD1 Protein (Lys309-Ser481) with a N-His tag was expressed in E. coli.

Specification

• Description: Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA7 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 23.4 kDa
  Accurate Molecular Mass: 23 kDa
• Protein length: Lys309-Ser481
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: OTUD1 OTU domain containing 1 [ Homo sapiens (human) ]
• Official Symbol: OTUD1
• Synonyms: OTUD1; OTU deubiquitinase 1; DUBA7; OTDC1; OTU domain-containing protein 1; DUBA-7; OTU domain containing 1; EC 3.4.19.12
• Gene ID: 220213
• mRNA Refseq: NM_001145373
• Protein Refseq: NP_001138845
• MIM: 612022
• UniProt ID: Q5VV17