Recombinant Human OTUB2, His-tagged

• Cat.No.: OTUB2-30525TH
• Product Overview: Recombinant full length protein, (amino acids 1-234) of Human OTUB2 with N terminal His tag; 254 amino acids, 29.4 kDa.

Specification

• Description: This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain.
• Protein length: 234 amino acids
• Conjugation: HIS
• Molecular Weight: 29.400kDa inclusive of tags
• Source: E. coli
• Tissue specificity: Widely expressed. Expressed at higher level in brain.
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 0.02% DTT, 10% Glycerol, 0.29% Sodium chloride
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMSETSFNLISEKCDILSILR DHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSY LESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFN AFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTS AFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQI TALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLY KTSHYNILYAADKH
• Sequence Similarities: Belongs to the peptidase C65 family.Contains 1 OTU domain.

Gene Information

• Gene Name: OTUB2 OTU domain, ubiquitin aldehyde binding 2 [ Homo sapiens ]
• Official Symbol: OTUB2
• Synonyms: OTUB2; OTU domain, ubiquitin aldehyde binding 2; C14orf137, chromosome 14 open reading frame 137; ubiquitin thioesterase OTUB2; FLJ21916; MGC3102
• Gene ID: 78990
• mRNA Refseq: NM_023112
• Protein Refseq: NP_075601
• MIM: 608338
• Uniprot ID: Q96DC9
• Chromosome Location: 14q32.13-q32.2
• Function: cysteine-type peptidase activity; omega peptidase activity; peptidase activity; ubiquitin-specific protease activity; ubiquitin-specific protease activity